Laboratory Analysis of Samples III - Molecular Analysis EBSP (APW)

Laboratory Analysis of Samples III (Molecular Analysis) for English Bachelor's study programme (APW)
Guarantor:Mgr. Ivo Papoušek, Ph.D.

Lectures

• Lecture 01- Introduction (2024)
• Lecture 02- Levels of molecular biology, methodical approaches (2024)
• Lecture 03 - Polymerase chain reaction (2024)
• Lecture 04 - RFLP, sequencing, hybridization (2024)
• Lecture 05 - Molecular analyses of RNA and proteins (2024)
• Lecture 06 - Specific molecular analyses in food hygiene and quality, diagnostics and medicine

Lectures will be uploaded according to the actual spring semester.

Practicals

• Practical 01- Introduction (2024)
• Practical 02- Biological data, databases, GLP, basics of working with NAs and proteins (2024)
• Practical 03 - Molecular analysis of meat mixture (2024)
• Practical 04 - Measuring concentration of isolated DNA, preparing mixture for multiplex PCR (2024)
• Practical 05 - Gel electrophoresis, restriction endonucleases (2024)
• Practical 06 - Criminology, forensic genetics (2024)
• Practical 07 - Evaluation of sequence data (2024)

Practicals will be uploaded according to the actual spring semester.

Exam:

• Exam topics spring semester 2024