Laboratory Analysis of Samples III - Molecular Analysis EBSP (APW)

for English Bachelor's study programme (APW)

Guarantor: Mgr. Ivo Papoušek, Ph.D.

Exam

•  Exam topics spring semester 2024

Lectures

•  Lecture 01  - Introduction (2025)
•  Lecture 02  - Levels of molecular biology, methodical approaches (2025)
•  Lecture 03  - Polymerase chain reaction (2025)
•  Lecture 04  - RFLP, sequencing, hybridization (2025)
•  Lecture 05  - Molecular analyses of RNA and proteins (2024)
•  Lecture 06  - Specific molecular analyses in food hygiene and quality, diagnostics and medicine

Lectures will be uploaded according to the actual spring semester.

Practicals

•  Practical 01- Introduction (2025)
•  Practical 02- Biological data, databases, GLP, basics of working with NAs and proteins (2025)
•  Practical 03 - Molecular analysis of meat mixture (2025)
•  Practical 04 - Measuring concentration of isolated DNA, preparing mixture for multiplex PCR (2025)
•  Practical 05 - Gel electrophoresis, restriction endonucleases (2025)
•  Practical 06 - Evaluation of sequence data (2025)
•  Practical 07 - Criminology, forensic genetics (2024)

Practicals will be uploaded according to the actual spring semester.